Product Description

REAGEN? Aflatoxin M1 Strip  Test  is a  qualitative  and rapid  lateral  flow assay  designed  to detect Aflatoxin M1 residues in raw commingled  cow’s  milk at 0.5ppb. This immunochromatographicassay eliminates the need for a heating block. This test is designed for field use orreference lab settings.The unique features of the kit are:

l          Rapidstrip test method – 10 minutes

l         Highaffinity antibody method that requires no heating step

l         Worksin cold milk

l         Nosample preparation

l         Highsensitivity

l         Highreproducibility

ProcedureOverview

Theassay  uses a competitive colloidal goldbased format. The milk sample  (200 μL)is added to a clear plastic reaction vessel, and used to resuspend thelyophilized reagents to a uniform pink color in the  bottom of the microtiterwells. The  milk  is incubated briefly (5  min) at roomtemperature to allow the Aflatoxin M1 binding protein on  the  gold particles  to engage  with any  Aflatoxin M1  antibiotic present in the milk. The  test strip  is  then inserted  into  the sample  well  with the  arrows  pointing downward  initiating capillaryflow  up the strip.  Any gold  particles  that are not  complexed  with antibiotics  present in the milk  will bind to the  Aflatoxin M1  antibiotic imprinted  at the  Test line  (T-line),  forming a signal  (red line)  at that  position.  If the  Aflatoxin M1  binding protein  on  the gold  particle  has engaged  with antibiotic presentin the milk sample, the gold particle will flow past the T-line and reach theControl line (C-line).  For  visual interpretation of the test  results, T-line signal intensity  that  is stronger than the  signal at  the  C-line indicates  a  negative result.  Signal  at the  T-line  which is  equal  or less intense  compared  to the  C-line  indicated the presence  of  Aflatoxin M1 antibiotics  in  the milk.  The greater the  reduction in  signal  intensity at  the T-line,  the greater the  concentration  of Aflatoxin M1 antibiotics present in the milk.